Types Of Cytometry Devices Used in Cell Culture
Cytometry is a process of assigning the number of cell characteristics through scientific experiments. The measurement involves developing cells using both bacterial and eukaryotic cells. The method includes the study of the shape and size of the cell at different phases. It takes place in the protoplasm of a cell, excluding the nucleus. Various scientific researches and medical diagnostics rely on Cytometry to identify cells in treating diseases. Before investing in a cytometry measurement, check out its types of devices and purposes.
Flow cytometry
Ever since the invention of the cell culture incubator in the 1950s, flow cytometry has become a popular technic for testing microbiological samples. This method enables the cell development procedure to reproduce microorganisms such as yeast and viruses and store biohazard samples. The biological lab requires sophisticated machines, such as the Coulter Counter, run by electrical power. In the incubation process, the type of fluorochromes stains the cells to obtain the characteristics of particular cells in the Cytometry.
Cell sorting
This method involves a device known as a fluorescence-activated cell sorter (FACS) that selects the cells by examining their specific characteristics. The device will sort the cell from blood under sterile conditions inside a container. If the cell is precarious, it is impossible to perform the test without budging or damaging its form. As a reason, there is a need for constant adherence in the equation for sorting the cell numbers based on their characteristics. The process needs vessels that swing the single-cell sample at separating the samples based on their characteristics.
Imaging cytometry
A traditional way to measure cell numbers is imaging cytometry, which brings fluorescence microscopy to the experiment. It involves the sampling of cells for syllable structure and multi-parameter fluorescence. Before putting the cell for examination, it will stain fluorochromes to be viewed through a standard hemocytometer. This process requires high-resolution lenses and a screen to capture and display the characteristics of the cell. Although it is the oldest form of Cytometry, modern labs rely on new technology for cell culture.
Mass Cytometry
As the name suggested, mass cytometry labels bulk cells with metal ion tags. The process allows top-rated examination by providing multiple flexible surgical instruments with a blunt end. This technic enables the development of similar organisms with the interaction of their genotype and the environment. And thus, the latest device in Cytometry is appropriate for complicated cell culture and immunology industries. The outstanding feature of mass Cytometry is the analysis of fine spay and ion atoms in the chamber.
When upgrading the lab for the next cell culture project, find a Cytometry reagent company that makes a landmark in selling flow cytometry staining buffer online. The company provides the latest devices necessitated in virology and cytology. Many enterprises in this industry rely on the best tools and technic to ensure an accurate reading.
Author’s Bio — The writer is an avid online blogger. This article is about flow cytometry staining buffer online.